Isolation and Optimization of Lipase Producing Bacteria from Oil Contaminated Soils
نویسندگان
چکیده
Lipolytic bacteria were isolated from oil contaminated soils and grown on tributyrin media containing 1% (w/v) olive oil. The isolate showing maximum activity was identified by following Berger’s manual. Different media parameters were optimized for maximal enzyme production. Peak lipase activity was observed for palm oil as carbon source, peptone as nitrogen source, at pH 7.0 and temperature at 37°C.
منابع مشابه
Isolation, Optimization, and Molecular Characterization of a Lipase Producing Bacterium from Oil Contaminated Soils
Lipases have many applications in biotechnology, thanks to their ability of acylglycerides hydrolysis. They alsp possess the unique feature of acting at the lipid-water interface, which distinguishes them from esterases. Commercially useful lipases are produced by microorganisms with the extracellular lipase being produced by many bacteria including Pseudomonas. The greatest production of lipas...
متن کاملIsolation, Optimization, and Molecular Characterization of a Lipase Producing Bacterium from Oil Contaminated Soils
Lipases have many applications in biotechnology, thanks to their ability of acylglycerides hydrolysis. They alsp possess the unique feature of acting at the lipid-water interface, which distinguishes them from esterases. Commercially useful lipases are produced by microorganisms with the extracellular lipase being produced by many bacteria including Pseudomonas. The greatest production of lipas...
متن کاملIsolation of lipase producing bacteria from olive extract to improve lipase production using a submerge fermentation technique
The tremendous mounting interest in lipase production is pertinent to the biotechnological applications that these enzymes offer. Lipase, belonging to the hydrolyze enzymes, is involved in copious biological functions including: hydrolysis insoluble triacylglycerol to mono and diacylglycerols and glycerol. Submerge fermentation technique was applied for the efficient production of this. Briefly...
متن کاملIsolation of lipase producing bacteria from olive extract to improve lipase production using a submerge fermentation technique
The tremendous mounting interest in lipase production is pertinent to the biotechnological applications that these enzymes offer. Lipase, belonging to the hydrolyze enzymes, is involved in copious biological functions including: hydrolysis insoluble triacylglycerol to mono and diacylglycerols and glycerol. Submerge fermentation technique was applied for the efficient production of this. Briefly...
متن کاملIsolation of lipase producing bacteria from olive extract to improve lipase production using a submerge fermentation technique
The tremendous mounting interest in lipase production is pertinent to the biotechnological applications that these enzymes offer. Lipase, belonging to the hydrolyze enzymes, is involved in copious biological functions including: hydrolysis insoluble triacylglycerol to mono and diacylglycerols and glycerol. Submerge fermentation technique was applied for the efficient production of this. Briefly...
متن کامل